Coding

Part:BBa_K3013001:Design

Designed by: Yasmin Kuhn   Group: iGEM19_Aachen   (2019-10-08)


TA2, polymer binding peptide


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 16
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 16
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Changed codon at position 19 - 21 from CAG to CAA to make it compatible with RFC 10.


Source

We've got the part's sequence from the following paper: https://doi.org/10.1002/bit.26481

References